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DOI:
食品研究与开发:2016,37(16):-
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壳聚糖固定化果胶酶澄清低度姜酒的工艺优化
(1.山东省莱芜市第一中学,山东莱芜271100;2.莱芜职业技术学院生物教研室,山东莱芜271100)
Optimization the Technology of Low-ginger Liquor Clarification by the Pectinase Immobilized with Chitosan
(1.Laiwu No.1 Senior High School Shandong Province,Laiwu 271100,Shandong,China;2.Biochemistry Staff Room,Laiwu Vocational Technology Institute,Laiwu 271100,Shandong,China)
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投稿时间:2015-09-19    
中文摘要: 采用壳聚糖固定化果胶酶澄清低度姜酒。在单因素试验的基础上,采用正交试验确定固定化果胶酶澄清低度姜酒的最优工艺为:固定化酶浓度为60 g/L,pH值为3.5,酶解温度和时间分别是45℃和12 h。在该工艺下低度姜酒透光率可达98.9%,所得低度姜酒稳定性好,壳聚糖固定化果胶酶重复使用7次后姜酒透光率仍保留有81.2%。固定化果胶酶比游离酶澄清低度姜酒效果好,稳定性提高。
中文关键词: 壳聚糖  固定化酶  果胶酶  低度姜酒  澄清
Abstract:Chitosan-immobilized pectinase was applied to clarify low-ginger liquor.Based on the result of single factor experiments,orthogonal experiment was performed.The optimizate conditions for charification of lowginger liquor were:immobilized enzyme amount 60 g/L,pH value 3.5,enzymolysis temperature 45℃,enzymolysis time 12 h,transmittance reached 98.9%,The transmittancy of low-ginger liquor was 81.2%after immobilized enzyme was repeatedly used seven times.The clarification effects of immobilized enzyme were better than free enzyme.
文章编号:201616019     中图分类号:    文献标志码:
基金项目:山东省科技发展计划项目(2011GGA12054);莱芜高新区科技创新与发展计划项目(201015)
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