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投稿时间：2015-08-10
投稿时间：2015-08-10
中文摘要: 采用化学共沉淀法制备Fe3O4纳米微粒，并对其粒径大小、磁响应性和磁分散性进行综合分析。利用反向悬浮包埋法，以Fe3O4纳米微粒为载体制备L-赖氨酸高分子微球，并将其运用于猪血清中蛋白的分离。以猪血清蛋白偶联率为指标，通过正交试验优化L-赖氨酸高分子微球作用于猪血清蛋白的最佳作用条件。结果表明：最佳作用条件为反应温度40℃，作用时间3h，血清用量20mL，在此条件下测得猪血清蛋白的偶联率达到18.59%。
Abstract:This experiment used chemical Co-precipitable to get Fe3O4particles，then analyzed its particle size，magnetic response and magnetic dispersion.Use Fe3O4nanoparticles as carriers for the preparation of L-lysine polymer microsphere by reverse suspension method，which was applied to the separation of pig serum protein.Coupled rate with the pig's serum was used as a target to optimize the technical conditions through the orthogonal experiments.The results showed that the best condition was as follow，the reaction temperature was 40℃，the reaction time was 3 h and the serum dosage was 20 mL，under these conditions the serum protein coupling rate could reach to 18.59%.
keywords: Fe3O4particles L-lysine pig'sserumproteins coupledrate
文章编号：201615012 中图分类号： 文献标志码：
基金项目:四川省大学生创新创业训练计划项目（201510623055）
| Author Name | Affiliation |
| LI He，XU Dan，LI Xiao-feng | School of Food and Biological Engineering，Xihua University，Chengdu 610039，Sichuan，China |
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