Abstract:A polysaccharide GFD-1 from Grifola frondosa fruiting bodies had been purified by sonication，water extraction，alcohol precipitation and Sephadex gel filtration chromatograp.By HPLC，GFD-1 was a kind of polysaccharide homogeneous components and its molecular weight was 29.574 kDa.The results of the color experiment，ultraviolet spectroscopy，infrared spectroscopy and atomic force microscope analysis illustrated that GFD-1 was a kind of non-starch α-pyran polysaccharide without reductive carbonyl，phenolic hydroxyl，free or a combination protein and nucleic acid；its sugar chain height was 0.5 nm-1 nm.GFD-1 inhibited proliferation of HepG2 cells in a dose-dependent manner，the IC50of GFD-1 was 94 μg/mL；it had no significant effect on the cell viability of humor normal liver L-02 cells.Meanwhile，in the presence of with GFD-1，HepG2 cells showed typical apoptotic morphological features through the AO/EB double staining.Flow cytometry analysis suggested that HepG2 cells were repressed at the phase S by GFD-1.These results showed that GFD-1 purified from Grifola frondosa possesses anti-tumor regulation effect，serving a reference basis to develop natural anticancer drugs and further analyse the anti-tumor mechanism of GFD-1.