Abstract:The effects of polysaccharides from Gynostemma pentaphyllum Makino（GMP）on the exhaustive exercise-induced hepatocyte apoptosis of rats was studied.The animal model of exhaustive exercise was used for study.Thirty two male Wistar rats were randomly assigned into quiet control group（QC），exhausting exercises control group（EC），low dose GMP+exhausting exercises group（LGE），high dose GMP+exhausting exercises group（HGE）.The expressions of Bcl-2 and Bax were detected by the immunohistochemical staining and image analyzer.The correlation between the ratio of Bcl-2/Bax and histiocyte apoptosis was analyzed.Meanwhile，main antioxidant enzyme activities and malondialdehyde （MDA）were detected by the spectrophotometric method.After GMP was provided by gavage，the expression of Bax decreased remarkably，and of Bcl-2 increased significantly，the ratio of Bax/Bcl-2 decreased remarkably.GMP could decrease MDA contents and increase superoxide dismutase（SOD），glutathione peroxidase（GPx），catalase（CAT）activities in liver of rats.GMP possessed protective effects on the exhaustive exercise-induced cell apoptosis and oxidative stress.