Abstract:The sterilization process is an important part of food processing，traditional thermal sterilization in killing spoilage microorganisms at the same time，can cause food color，flavor and nutrients varying degrees of change and loss.There fore，non-thermal sterilization technology emerges as the times require and continue to develop，where electric field sterilization technology utilizes high-voltage pulse applied to the material，microbial membrane electrical breakdown in the strong electric field，produce irreparable perforation，lead to microbial inactivation，and reach the effect of sterilization.In this study，the high electric field strength caused by alternating current was used to process the sterilization of liquid yeast，the effects of temperature，sterilization time，liquid velocity and electric field strength of the raw materials on the sterilization were studied，in order to find the best sterilization process parameters，as to provide theoretical basis for food sterilization technology.Experiment showed that the optimum parameters of the sterilization process was：raw material liquid temperature of 40℃，sterilization time of 80 s，liquid flow rate of 100 mL/min，the electric field intensity of 10 kV/cm，the optimum technology was adopted to improve the sterilization，the death rate of yeast bacterium reached to 95.5%.This study showed that the ac electric field sterilization equipment could effectively kill yeast，had certain ability of sterilization.