Abstract:To investigate the anti-angiogenic effect of Boschniakia rossica extract mainly consisted of iridoid glucoside（IGBR）in H22-bearing mice,mice bearing H22hepatoma cells were established and treated with IGBR for 10 days.The pathological changes were detected with HE staining，the microvessel densities（MVD）were detected using immunohistochemical assay，and the protein expression of hypoxia-inducible factor（HIF）-1α，vascular endothelial growth factor（VEGF）and vascular endothelial growth factor receptor（VEGFR）-2 was detected by western blot method.As compared with the model group，IGBR treatment reduced the cell proliferation，aggravated the necrosis of transplanted tumor tissues of H22hepatoma cells.In addition，the administration with IGBR decreased MVD of tumor tissue，and down-regulated the protein expression of HIF-1α，VEGF and VEGFR-2 in the transplanted tumor tissue.Taken together，IGBR could exhibit anti-angiogenic effect against H22hepatoma cells in mice，and its mechanism was probably related to down-regulation of HIF-1α and VEGF proteins.