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投稿时间：2016-03-04
投稿时间：2016-03-04
中文摘要: 以荧光微球作为新型标记物，采用EDC/NHS两步法偶联，制备荧光微球链霉亲合素复合物。复合物颗粒均一、性质稳定。以其作为探针，建立检测牛奶中生物素含量的荧光免疫层析法。本方法特异性良好，最低检出限低至3 ng/mL，线性范围5 ng/mL～100 ng/mL，回收率90%～110%，精密度CV＜10%；与微生物法对比，测值相关系数为0.975。
Abstract:A composition with a character and uniform size which contained fluorescentmicrospheres as a novel lable was obtained by an EDC/NHS two-step method.The biotin in milk was detected by the immunofluorescence chromatography which had this compositoin as a probe.The method showed good specificity with the detection limitof3 ng/mL，the linear range of 5 ng/mL-100 ng/mL，the recovery of90%-110%and the precision CV less than10%；the correlation coefficient of measuring value was 0.975 compared with microbiological method.
keywords: fluorescentmicrospheres probe detection limit
文章编号：201612032 中图分类号： 文献标志码：
基金项目:
| Author Name | Affiliation |
| LIU Hai-ying | Food Engineering Departmentof Inner Mongolia Business&Trade VocationalCollege，Hohhot010070，Inner Mongolia，China |
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