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投稿时间:2015-07-06
投稿时间:2015-07-06
中文摘要: 通过生物排杂法将花生粕中的淀粉酶解水溶,建立热榨花生粕蛋白生物酶法的制备工艺。以淀粉去除率为考察指标,进行酶解工艺的单因素试验。根据单因素试验结果,选取料液比、时间、酶用量进行三因素三水平的响应面优化,最佳条件为:加酶量0.55%,料液比为1∶8(g/mL),pH 6.0,温度60℃,酶解时间为1.0 h;制备的花生蛋白纯度为81.38%,达到了分离蛋白水平。
Abstract:A novel preparation method of peanut protein was established by enzymatic hydrolysis of starch in hot pressed meals.Starch removal rate as index,the single factor experiments of enzymatic hydrolysis technology were carried out.Based on above results,the response experiment for three factors(solid-liquid ratio,time,enzyme dosage)and three levels was designed.The optimal conditions were as follows:enzyme dosage of 0.55%,solid-liquid ratio of 1∶8(g/mL),pH 6.0,60℃,reaction time of 1.0 h.The purity of preparation peanut protein reached 81.38%as well as that of separation protein.
文章编号:201611019 中图分类号: 文献标志码:
基金项目:国家“863”计划项目(2013AA102206)
作者 | 单位 |
马治良1,2,徐晓倩3,戴明涛4,徐同成1,高冠勇2,陈宁2,陶海腾1,* | 1.山东省农业科学院农产品研究所,山东济南250100;2.山东金胜粮油集团,山东临沂276600;3.莒南县农业综合开发办公室,山东临沂276600;4.莒南县农业局,山东临沂276600 |
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