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食品研究与开发:2016,37(10):-
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瑞士乳杆菌AJT产细菌素的发酵条件优化
(1.包头市农畜产品质量安全监督管理中心,内蒙古包头014010;2.江苏汉邦科技有限公司,江苏南京223005;3.内蒙古农业大学,内蒙古呼和浩特010018)
Optimization of Fermentation Conditions for Bacteriocin Production by Lactobacillus helveticus AJT
(1.Baotou Livestock Products Quality and Security Supervisin and Management Center,Baotou 014010,Inner Mongolia,China;2.Jiangsu Hanbang Technology CO.,LTD,Nanjing 223005,Jiangsu,China;3.College of Food Science and Engineering,Inner Monglia Agricultural University,Hohhot 010018,Inner Mongolia,China)
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投稿时间:2015-06-06    
中文摘要: 瑞士乳杆菌AJT是一株广谱抗菌活性菌株,对革兰氏阳性、革兰氏阴性细菌和真菌均有较好的抑制作用。通过优化试验确定其最优的培养基成分为:乳糖15.0 g/L、酵母浸出粉7.0 g/L、胰蛋白胨6.0 g/L、牛肉浸膏12.0 g/L、乙酸钠7.0g/L、柠檬酸钠7.0 g/L、磷酸氢二钾2.0 g/L、硫酸镁200 mg/L、硫酸锰40 mg/L,吐温-80 1.0 g/L,起始pH6.5;最佳培养条件:接种量2.0%,37℃培养20h。与未优化发酵条件相比,粗提取的瑞士乳杆菌AJT细菌素对蜡状芽孢杆菌的抗菌活性提高了53.8%,对黄色镰孢的抗菌活性提高了41.5%,对大肠杆菌的抗菌活性提高了68%。
中文关键词: 瑞士乳杆菌  细菌素  发酵条件  优化
Abstract:Lactobacillus helveticus AJT is a broad antibacterial lactobacillus has strong antibacterial activity against Gram-positive,Gram-negative and fungi strains.Single factor and orthogonal tests were conducted to de termine the media for optimum antibacterial activity culture,and the optimized ingredient was:lactose 15.0 g/L,yeast extract 7.0 g/L,peptone 6.0 g/L,beef extract 12.0 g/L,sodium citrate hydrate 7.0 g/L,sodium acetate 7.0 g/L,K2HPO42.0 g/L,MgSO4·7H2O 200 mg/L,MnSO4·5H2O 40 mg/L and tween-80 1.0 g/L,with initial pH at 6.5,the optimum incubation parameters were incubated at 37℃for 20 h with 2%(volume fraction)concentration.Its antibacterial activity of the extract increased 53.8%,41.5%and 68%acting on Bacillus cereus ,Fusarium culmorum and Escherichia coli respectively compared to the unoptimized fermentation condition.
文章编号:201610043     中图分类号:    文献标志码:
基金项目:内蒙古自治区应用技术研发资金计划项目(20130438);内蒙古自然科学基金面上项目(2014MS0358)
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