Abstract:Evaluation of GB 4789.11-2014＜National food safety standard Food microbiological examination：β-hemolytic streptococcus test＞in modified tryptone soy broth medium quality.a total of three domestic（labeled A，B，C）and foreign（labeled D）a culture based manufacturer of tryptone soya broth culture base（commercial dehydration synthetic culture medium）and the corresponding additives，use target bacteria（βhemolytic streptococcus ATCC21059）and non target bacteria（Escherichia coli ATCC25922，Staphylococcus aureus ATCC25923），according to the GB4789.28-2013＜National food safety standards Food microbiological examination Quality requirements for media and reagents＞in selective enrichment culture based qualitative test method for quality evaluation.At the same time without additives test.The A，B and C three modified tryptone soy broth culture medium target bacteria turbidity values are 0 and non target bacteria turbidity value of 0；without reagent medium target bacteria turbidity values are 0，non target bacteria turbidity value of 0.Foreign D improvement of the pancreatic tryptone soya broth culture medium target bacteria turbidity value is 2，non target bacteria turbidity value of 0；without reagent culture based target bacteria turbidity value of 2，non target bacteria turbidity values for 1.Domestic A，B，C three modified tryptone soy broth medium quality assessment that is tryptone soya broth culture medium（without additives）based problem；domestic tryptone soya broth culture medium quality needs to be improved.