Abstract:Starch was extracted from highland barley flours assisted by cellulose.In order to find the best conditions of enzymatic hydrolysis，with the starch extraction yield as the evaluation index，an orthogonal test was adopted to investigate added-amount of cellulase，enzymatic time，enzymatic temperature and pH value based on the results of single-factor tests.According to the best extraction conditions obtained，ultrasonic pilot enlarge experiment was carried out.The result of the orthogonal optimization test showed that added-amount of cellulase，enzymatic temperature，as well as the interaction between enzymatic time and enzymatic temperature have significant effects on the starch extraction yield.The best extraction conditions would be：added-amount of cellulase was 100 U/g，enzymatic temperature was 45℃，enzymatic time was 6 h and pH value was 4.8，under which the starch extraction yield from highland barley was 80.02%.The result of the ultrasonic pilot enlarge experiment indicates that the best extraction conditions obtained by the orthogonal optimization test were stable.