本文已被：浏览 320次 下载 0次
投稿时间：2015-01-22
投稿时间：2015-01-22
中文摘要: 以长白山榛子分离蛋白为原料，采用碱性蛋白酶制备抗氧化肽，通过Box-Behnken中心组合试验确定最佳水解工艺条件为：酶解温度50℃、加酶量8 000 U/g、底物浓度5.0%、pH9.5。对该条件制备的抗氧化肽进行活性研究，结果显示随着浓度的升高，榛子抗氧化肽在4mg/mL时对ABTS自由基和DPPH清除率均达到100%，8mg/mL时对·OH清除率和总还原能力分别达到85.46%和0.859，12mg/mL时，对Fe2+螯合率达到99.3%。
Abstract:In this experiment，hazelnut nut protein isolated was used as raw material，hydrolyzed by Alcalase to prepare antioxidant peptide.Through Box-Behnken method，the optimal hydrolysis conditions were determined as hydrolysis temperature of 50℃，Alcalase concentration of 8 000 U/g，substrate concentration of 5.0%，hydrolysis pH of 9.5.Assess the antioxidant potential，with the increasing of the peptide solution concentration，scavenging capacities against ABTS and DPPH were 100%at 4 mg/mL，scavenging capacities against hydroxyl radical and reducing power were 85.46%and 0.859 at 8 mg/mL，Ferrous ions chelating capacity of hazelnut antioxidant peptide was 99.3%at 12 mg/mL.
keywords: hazelnut protein isolated antioxidant peptide enzymatic hydrolysis optimization antioxidant activity
文章编号：201610001 中图分类号： 文献标志码：
基金项目:国家“863”计划项目（2013AA102206）
引用文本：
津ICP备20000603号-1