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投稿时间：2016-02-22
投稿时间：2016-02-22
中文摘要: 建立以 QuEChERS EMR-Lipid 为前处理测定动物源性食品中 8 种 β-受体激动剂的 LC-MS/MS 方法。 样品经 粉碎混匀后加入 pH 为 5.2 乙酸-乙酸铵溶液， 以 β-盐酸葡萄糖醛酸苷酶/芳基硫酸酯酶酶解， 酶解液经 QuEChERS EMR-Lipid 净化后，取乙腈层与 0.1 %的甲酸水溶液混合上 LC-MS/MS 进行分析。8 种 β-受体激动剂在测定浓度范围内 线性关系良好，各组分相关系数 R2 >0.998，加标回收率范围 79.1 %~94.8 %，方法精密度范围 5.4 %~9.4 %，测定各组分 β-受体激动剂检出限范围 0.2 μg/kg~0.4 μg/kg，定量限范围 0.5 μg/kg~1.0 μg/kg。
中文关键词: β-受体激动剂 高效液相色谱串联质谱 QuEChERS 脂质去除 肉制品
Abstract:A method with QuEChERS EMR-Lipid pretreatment for the determination of 8 β-agonists in animal tissues by liquid chromatography-tandem mass spectrometry（LC-MS/MS）was established. The crushed sample was added to sodium acetate （pH 5.2） and enzymatic hydrolysis by β-Glucuronidase/Arysulfatase. The sample was purified by QuEChERS EMR-Lipid and took the acetonitrile mixed with 0.1 % formic acid solution， then detected by LC-MS/MS. The 8 kinds of β-agonists showed good linear relationship between the concentration range， components correlation coefficient R2＞0.998， standard addition recovery range between 79.1 %-94.8 %， precision range 5.4 %-9.4 %， LOD components range 0.2 μg/kg-0.4 μg/kg and LOQ components range 0.5 μg/kg-1.0 μg/kg.
keywords: β-agonists LC-MS/MS QuEChERS EMR-Lipid meat
文章编号：201609042 中图分类号： 文献标志码：A
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