Abstract:A quantitative analysis method of multi-components with single marker （QAMS） was established and validated to simultaneously determine six Soybean isoflavones （ISO）in health foods ， which can solve the dilemma of natural chemical reference substances are expensive and unavailable. The samples were ultrasonic-assisted extracted with methanol， the column was Waters XbridgeC18 （150 mm×4.6 mm，3.5 μm），using 2 % acetic acid and acetonitrile as the mobile phase with gradient elution， 260 nm and with DAD detection was used. The component to be measured were confirmed by DAD spectrum， reference chromatogram and the relative re－ tention time of genistin. The relative correction factors （RCF） of the other five soybean isoflavones were deter－ mined within the linear ranges， the RCF had a good reproducibility in various instruments， chromatographic columns（RSD = 0.4 % -3.5 %）. According to their RCF， six ISO in health food could be quantified.The results of QAMS method were validated by comparing with that of external standard method by quantified three different sources of health food ， and the relative average deviation of the two method were 0.3 %-5.8 %. The method can be used for the quantitative analysis and quality evalution for ISOs and its related health food.