Abstract:Taking Metschnikowia pulcherrim as test material， the study on the influence of growth and antibac－ terial effect of antagonistic yeast by adding different carbon sources， nitrogen sources， and protective agents un－ der cold stress， in order to clarify ways to improve biocontrol effect of antagonistic yeast. The results showed that sucrose as carbon source， yeast extract as nitrogen source was beneficial for growth of M. pulcherrima under cold stress condition， the antibacterial effect in vitro was best， the biocontrol efficacy of M. pulcherrima could significantly improve by adding 0.1 % CaCl2 and 2 % trehalose.