Abstract:To analyze and identify the chemical constituents in herbal tea by HPLC-Q-TOF-MS. The samples were extracted with methanol， separated by the Diamonsil C18 column with a gradient elution of acetonitrilewater containing 0.1 % formic acid. Positive and negative ion mode electrospray ionization and high-resolution quadrupole time of flight mass spectrometry was employed for qualitative analysis. Based on the MS/MS analy－ sis， comparing references and relevant literature dates， forty-three compounds of herbal tea were identified， mainly including organic acids and flavonoids. Furthermore， all of the constituents were surveyed and classified according to their medicinal materials derivation. The results showed that HPLC-Q-TOF-MS could elucidate the main components in herbal tea in a more comprehensive way and the method established was simple， fast and reliable， which laid the foundation for quality control and functional components studies.