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投稿时间:2015-02-01
投稿时间:2015-02-01
中文摘要: 研究超声波辅助提取莲子心总黄酮的最佳工艺条件。单因素试验考察乙醇体积分数、超声温度、超声功率、料 液比、超声时间对总黄酮提取得率的影响,在单因素试验基础上,采用 Box-Benhnken 响应曲面法考察乙醇体积分数、料 液比、超声温度及其交互作用对总黄酮得率的影响。利用软件 Design Expert 6.0.1 对试验数据进行回归分析,得到二次多 项式回归方程预测模型。结果表明,莲子心总黄酮的提取工艺最佳条件为乙醇体积分数 80 %,料液比 1 ∶ 16(g/mL),超声 温度 50 ℃,超声功率 120 W,超声时间 20 min,此条件下总黄酮的提取得率为 3.071 5 %,与预测值基本一致。
Abstract:The study aimed at optimizing the condition for extraction of total flavonoids from lotus plumule by means of unrasonic-assisted technique. The effects of ethanol valume fraction, ultrasonic temperature, ultra- sonic power, solid-liquid ratio, ultrasonic time on the yield of total flavonoid were discussed in the single factor experiment. Based on the single factor experiment, effects of ethanol valume fraction , solid-liquid ratio, ultra- sonic temperature and their interactions on the yield of total flavonoid were analyised by Box-Benhnken re- sponse method. The predictive model of polynomial regression equation was analysed by Design Expert software (6.0.1). The result showed that the optimum extraction of total flavonoids from lotus seed core were: ethanol val- ume fraction 80 %, solid-liquid ratio 1 ∶ 16(g/mL), ultrasonic temperature 50 ℃, ultrasonic power 120 W, ul- trasonic time 20 min, under this conditions the extraction of total flavonoids was 3.071 5 %, consistent with the predicted values.
文章编号:201608031 中图分类号: 文献标志码:A
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