本文已被：浏览 1377次 下载 694次
投稿时间：2014-10-25
投稿时间：2014-10-25
中文摘要: 立 HPLC 测定覆盆子中椴树苷和山奈酚含量的方法。采用高效液相色谱法，SHISEIDO C18 色谱柱（4.6 mm × 250 mm，5 μm）。流动相为甲醇—0.1 %磷酸水（52 ∶ 48，体积比），流速 1 mL/min，柱温 25 ℃，检测波长 366 nm。覆盆子中椴 树苷、山奈酚分离效果良好，线性范围分别为 3.2 μg/mL～160 μg/mL 和 0.168 μg/mL～8.4 μg/mL。该方法准确灵敏、稳定可 靠，可用于覆盆子的质量控制。
Abstract:To establish a HPLC method for the determination of tiliroside and kaempferol in rubus chingii hu. The method of HPLC was used to determine the content of tiliroside and kaempferol in rubus chingii hu.The method was established on SHISEIDO C18 column（4.6 mm × 250 mm， 5 μm） with the mixture of methanol - 0.1 %H3PO4 water（52 ∶ 48） as the mobile phase. The flow rate was 1 mL/min， and the column temperature at 25 ℃， the detection wavelength was set at 366 nm. This method had a good separation performance for tiliroside and kaempferol in rubus chingii hu， and the linear range were 3.2 μg/mL-160 μg/mL and 0.168 μg/mL-8.4 μg/mL，respectively. This method is accurate， sensitive， stable and reliable， can be used in the quality con－ trol of raspberry.
keywords: rubus chingii hu tiliroside kaempferol HPLC
文章编号：201607034 中图分类号： 文献标志码：A
基金项目:江西省科技攻关计划项目《覆盆子的炮制及其炮制品质量标准的研究》（赣科发计字[2006]38）
| Author Name | Affiliation |
| PING Yu-hui， LI Cai-li， XIE Yi-hui* | Jiangxi University of Traditional Chinese Medicine， Nanchang 330004， Jiangxi， China |
引用文本：
津ICP备20000603号-1