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食品研究与开发:2016,37(7):133-135
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柏实中黄曲霉毒素的HPLC-FD法测定
谢昕1,赵国欣2
(1. 河南职业技术学院, 河南 郑州 450046; 2. 中州大学, 河南 郑州 450044)
Determination of Aflatoxins in Biotaorientalis by HPLC-FD Detection
XIE Xin1, ZHAO Guo-xin2
(1. Henan Polytechnic, Zhengzhou 450046 , Henan, China; 2. Zhongzhou University, Zhengzhou 450044, Henan, China)
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投稿时间:2015-09-30    
中文摘要: 建立高效液相色谱测定柏实中黄曲霉毒素的方法,样品提取液经免疫亲和柱净化和光化学柱后衍生化,荧光 检测器检测,黄曲霉毒素 G2、B2 在 1.25 pg~50 pg 范围内,黄曲霉毒素 G1、B1 在 5 pg~200 pg 范围内线性关系良好,回收率 79.29 %~102.53 %。
中文关键词: 柏实  黄曲霉毒素  高效液相色谱
Abstract:This study had established a HPLC-FD method for determining the concentration of aflatoxins in Biotaorientalis. The sample extraction solution was purified by immune affinity column and derived with post-column photochemical. The aim components were detected by fluorescence detector. The linear range of the standard curve of aflatoxins G2 and B2 were 1.25 pg-50 pg, and those of aflatoxins G1 and B1 were 5 pg-200 pg. The recoveries were 79.29 %-102.53 %.
keywords: Biotaorientalis  aflatoxins  HPLC
文章编号:201607032     中图分类号:    文献标志码:A
基金项目:2015年度河南省重点科技攻关项目(152102310130); 2015年河南职业技术学院科研基金项目(2015HZK05)
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