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食品研究与开发:2016,37(6):43-49
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腰果蛋白的提取及功能特性研究
叶婧1,曾育蒙2,王秀丽1,*,李建平1
(1.广西高校食品安全与检测重点实验室 桂林理工大学化学与生物工程学院, 广西 桂林 541004;2.深圳爱康生物科技有限公司,广东 深圳 518131)
The Study on Protein Extraction from Cashew Nut and Its Functional Properties
YE Jing1, ZENG Yu-meng2, WANG Xiu-li1,*, LI Jian-ping1
(1.Guangxi Colleges and Universities Key Laboratory of Food Safety and Detection, College of Chemistry and Bioengineering, Guilin University of Technology, Guilin 541004, Guangxi, China; 2.Aikang Medtech CO.,LTD., Shenzhen 518131, Guangdong, China)
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投稿时间:2015-01-12    
中文摘要: 以腰果为材料,以碱提酸沉法提取腰果中的蛋白,确定最优的提取参数,并探讨了腰果蛋白的功能性质。通过 正交试验结果可知,提取腰果蛋白影响因素重要性依次为:料液比>提取时间>提取液 pH>温度;最佳提取工艺条件:提 取时间 2 h,提取液 pH8.5,料液比 1 ∶ 15(g/mL),温度 55 ℃,腰果蛋白提取率为 85.25 %。对腰果蛋白功能性质的研究表 明,腰果蛋白的等电点 pI 为 4.6;温度在 55 ℃时,蛋白质的溶解度达到最大;在浓度为 0~0.9 mol/L 的 NaCl 溶液中,腰果 蛋白出现盐溶现象,NaCl 溶液浓度达到 1.5 mol/L 时,则产生盐析现象;30 ℃时,腰果蛋白持水力为 3.34 g/g,随着温度的 升高,腰果蛋白持水力逐渐下降;随着 NaCl 浓度的增加,腰果蛋白持水力先增大后减小,NaCl 浓度为 0.9 mol/L 腰果蛋 白持水力最大达到 3.66 g/g;腰果蛋白在温度 30 ℃时持油力为 1.30 g/g,随着温度的升高腰果蛋白的持油性逐渐降低。
中文关键词: 腰果  提取  优化  腰果蛋白  功能性质
Abstract:It focused on the method which called alkali extraction and acid precipitation , the optimized extrac- tion parameters and its functional properties from the protein of cashew nut. Through the orthogonal experiment, the order of the extraction factors was as follows: solid -liquid ratio >time >pH >temperature, the optimized cashew nut protein extraction parameters was: extraction at 55 ℃ for 2 h with solid-liquid ratio 1 ∶ 15(g/mL), extraction buffer pH 8.5.Through the study of cashew nut protein functional properties, cashew nut protein iso- electric point pI=4.6,Temperature at 55 ℃ the solubility of protein dissolvability was the maximum. In the con- centration of NaCl solution 0-0.9mol/L, cashew nut protein appeared phenomenon of salt soluble, when NaCl solution concentration reached 1.5mol/L was developing salting-out. At 30 ℃ cashew nut protein water-retain- ing capacity was 3.34 g/g, with the increase of temperature, cashew nut protein water-retaining capacity gradu- ally decreases. Cashew nut protein water-retaining capacity firstly increased and then reduced with the concen- tration of NaCl solution increase, concentration of NaCl at 0.9 mol/L cashew nut protein water-retaining capac- ity was 3.66 g/g. At 30 ℃ protein oil-holding capacity was 1.30 g/g, as the temperature rises cashew nut protein oil-holding capacity gradually reduced.
文章编号:201606011     中图分类号:    文献标志码:A
基金项目:广西高校食品安全与检测重点实验室资助项目;广西自然科学基金资助项目(2015GXNSFAA139094)
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