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投稿时间：2015-11-02
投稿时间：2015-11-02
中文摘要: 建立高效液相法色谱测定人参果蔬酵素中的人参皂甙 Rg1、Re、Rb1、Rc、Rb2、Rd 的含量，用于评价产品的优劣。 色谱条件：DiamonsilC18（250 mm×4.6 mm，5 μm）色谱柱，流动相为乙腈与 0.05 mol/L 磷酸二氢钾水溶液，流 速 1 mL/min，梯度洗脱检测波长为 203 nm。该方法对人参皂甙 Rg1、Re、Rb1、Rc、Rb2、Rd 具有良好的线性，相关系数在0.999 3～0.999 9 之间；重复性和精密度良好，样品加标回收率在 92.00 %～101.50 %之间。RSD 小于 5%；检出限低。该方法 线性范围广，准确性和精密度良好，操作较简便，结果满意，能满足日常检验要求。
Abstract:To determine the content of panaxosides，such as Rg1、Re、Rb1、Rc、Rb2、Rd in enzyme of ginseng and fruit and vegetable，to evaluate the quality of the products， the HPLC method was established. Chromatographic conditions：Diamonsil C18 （250 mm×4.6 mm，5 μm）chromatographic column，flow phase for acetonitrile and 0.05 mol/L KH2PO4 aqueous solution，flow rate was 1 mL/min，gradient elution detection wavelength at 203 nm. The method had good linear correlation coefficient Rg1、Re、Rb1、Rc、Rb2、Rd and the correlation coefficient was between 0.999 3 and 0.999 9. The recovery rate was between 92 % and 101.50 %.RSD was less than 5 %，the detection limit was low. The method has a wide linear range，good accuracy and precision，the operation is simple，the results are satisfactory，can meet the daily inspection requirements.
文章编号：201605027 中图分类号： 文献标志码：A
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