Abstract:The green tea，obtained from tea-producing areas of the Tongcheng County of Hubei Province，was chosen as raw material. The optimum conditions were carefully studied in the process of assisted extraction of tea polyphenols from mixture of cellulose and pectinase. The impact of enzyme concentration，extraction time，extraction temperature and pH on tea polyphenols extraction rate was analyzed by single factor and orthogonal experiments，which was used to optimize the design of tea polyphenols extraction process conditions. The results showed that pH and cellulose significant could huge impact on the extraction of tea polyphenols. The optimal extraction conditions were cellulose 200 U/mL，the pectinase 300 U/mL，extraction time 50 min，extraction temperature 40 ℃，pH 5.0. The polyphenols extracted under optimum conditions was 23.54 % and the DPPH free radical clearance rate was 30 %，which showed excellent antioxidant properties.