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食品研究与开发:2016,37(5):72-76+217
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酶法制备鱿鱼皮胶原蛋白肽工艺的条件优化
刘振锋1,2,戴圣佳2,黄小鸣2,吕卫金2,陈士国1,刘东红1,叶兴乾1,*
(1. 浙江大学 馥莉食品研究院,浙江 杭州 310058;2. 浙江宇翔生物科技有限公司,浙江 杭州 310024)
Optimization of Enzymatic Preparation of Collagen Peptides from Squid Skin
LIU Zhen-feng1,2,DAI Sheng-jia2,HUANG Xiao-ming2,L譈 Wei-jin2,CHEN Shi-guo1,LIU Dong-hong1,YE Xing-qian1,*
(1. Fuli Institute of Food Science,Zhejiang University,Hangzhou 310058, Zhejiang, China;2. Zhejiang Yuxiang Biotech Co., Ltd.,Hangzhou 310024, Zhejiang, China)
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投稿时间:2014-12-03    
中文摘要: 研究了鱿鱼皮胶原蛋白肽的酶解加工工艺条件。在单因素试验基础上,通过正交试验的方法对碱性蛋白酶、木 瓜蛋白酶及风味蛋白酶制备鱿鱼皮胶原蛋白肽工艺进行优化。优化的提取工艺条件如下:先用 0.3 %蛋白酶在 pH10.5、 温度 47.5 ℃条件下水解 4 h,再用 0.4 %木瓜蛋白酶复合风味蛋白酶按 1 ∶ 2(质量比)的比例在 pH 7.5、温度 50 ℃的条件 下继续水解 4.5 h,结果酶解液的水解度可达 28.89 %、DPPH·清除率为 88.24 %,多肽转化率为 49.74 %,同时 84.56 %的 胶原蛋白肽分子量分布介于 180 Da~2 000 Da 之间。在优化的酶解条件下,可以得到较好品质的胶原蛋白肽产品。
中文关键词: 鱿鱼皮  胶原蛋白肽  酶法  正交试验
Abstract:The enzymatic method of collagen peptides from squid skin was studied. One-factor-at-a-time and orthogonal array methods were used to investigate the optimization of enzymatic preparation of collagen peptides from squid skin. The optimum extraction conditions:firstly,alcalase hydrolyzed for 4 h under the conditions of 47.5 ℃,pH 10.5 and enzyme concentration 0.3 %,then papain combined flavorzyme (1 ∶ 2,quality ratio) hy鄄 drolyzed for 4.5 h under the conditions of 50 ℃,pH 7.5 and enzyme concentration 0.4 %,then the peptides max鄄 imum DH was 28.89 %,the DPPH·scavenging rate was 88.24 % and recovery ratio of peptides was 49.74 %,in addition,the content of the collagen peptides had a molecular weight distribution of 180 Da-2 000 Da was 84.56 %. Result showed that the qualified peptides could be made under the optimized conditions.
文章编号:201605018     中图分类号:    文献标志码:A
基金项目:杭州市“雏鹰计划”(20131131k132);富阳市科研攻关专项(2014NK001);国家自然科学基金(31301417)
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