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食品研究与开发:2016,37(5):36-41
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内蒙紫草总黄酮提取工艺及其抗氧化活性研究
林海桢,施胜英,李舒婕,周溦,林敬明*
(南方医科大学珠江医院 药剂科,广东 广州 510282)
Study on Extraction Process and Antioxidant Activity of Total Flavonoids from Arnebia guttata Bunge
LIN Hai-zhen,SHI Sheng-ying,LI Shu-jie,ZHOU Wei,LIN Jing-ming*
(Department of Pharmacy,Zhujiang Hospital of Southern Medical University,Guangzhou 510282,Guangdong, China)
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投稿时间:2015-12-17    
中文摘要: 以内蒙紫草总黄酮得率为指标,在单因素试验基础上,采用响应面法优化超声波辅助提取工艺,并以芦丁为对 照,测定内蒙紫草总黄酮的抗氧化活性。结果表明,响应面试验获得的内蒙紫草总黄酮的最佳提取工艺为乙醇体积分数 58.57 %,料液比 1 ∶ 43.33(g/mL),超声时间 3.33 h,此时总黄酮得率 4.94 %。
Abstract:The objects of this study were to optimize extraction process and to research in vitro antioxidant activity of total flavonoids from Arnebia guttata Bunge. The extraction technology was optimized by single factor experiment and response surface methodology and the antioxidant effect was determined with rutin as positive control. The results had indicated that the extraction optimum technological conditions were the solid -liquid ratio 1 ∶ 43.33 (g/mL),the concentration of ethanol 58.57 %,the extracting time 3.33 h,and the yield of total flavonoids was 4.94 %.
文章编号:201605010     中图分类号:    文献标志码:A
基金项目:广东省自然科学基金项目(S2013010014796);海珠区科普计划项目(2014HZKP-DS-2)
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