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投稿时间：2014-11-07
投稿时间：2014-11-07
中文摘要: 以绿茶为原料，采用纤维素酶、果胶酶和漆酶组成的复合酶提取绿茶中的茶氨酸，并采用D-最优混料设计优化提取工艺。由建立的数学模型可知，复合酶提取茶氨酸的最佳工艺条件为茶叶浓度100 g/L，纤维素酶添加量0.657 %，果胶酶添加量0.543 %，漆酶添加量0.600 %，酶解pH 5.6、酶解温度50℃，酶解时间2 h。经过验证试验可知，在最优酶法提取工艺条件下茶氨酸的提取率可达到极大值0.421 %。
Abstract:Cellulase，pectinases and laccase were simultaneously used for the extraction of theanine from green tea in the present study，and the extraction process was optimized by D-optimal mixture design. On the basis of establishing mathematical model，the optimal extraction conditions for theanine were a material/liquid ratio of 100 g/L，extract at pH 5.6，55℃and for 2 h with 0.657 % cellulase，0.543 % pectinases and 0.600 % laccase. Under these conditions，a maximum total pectinases yeild efficiency of 0.421 % was observed by the verification test.
keywords: compound enzyme theanine mixed design
文章编号：201604016 中图分类号： 文献标志码：
基金项目:
| Author Name | Affiliation |
| YUE Kun，LIU Peng，SUN Yong-min | Tianjin Modern Vocation Technology College，Tianjin 300350，China |
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