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投稿时间：2014-10-14
投稿时间：2014-10-14
中文摘要: 选择 pH=7.2 的磷酸缓冲液为浸提剂从富硒黄豆中提取可溶性硒蛋白，研究了液料比、提取温度、提取时间对 黄豆蛋白提取率的影响。在单因素试验的基础上，根据 Box-Benhnen 的中心组合试验设计原理，采用三因素三水平的响 应面分析法确定了富硒黄豆中硒蛋白提取的最佳工艺：液料比 11 ∶ 1（mL/g）；提取温度 44 ℃；提取时间 96 min；在此条件 下，可溶性硒蛋白的提取率可达到 76.03 %。进一步研究表明：富硒黄豆中不仅含有硒蛋白，而且还含有大量的其他有机 硒化合物。
Abstract:Soluble selenium-containing protein was extracted from selenium-enriched soybean by PBS （pH=7.2）.The effects on protein extraction rate by the factors， such as liquid/ material ratio，temperature and extract time were studied. According to the single factor experiments，the optimum of extract process for selenium -enriched soybean protein was determined through Box-Benhnken central composite design and response surface methodology. The result showed that optimal condition of extraction were： liquid/material ratio 11 ∶ 1（mL/g）； extraction temperature 44 ℃ ； extraction time 96 min.Under the optimized extraction conditions， the yield of soluble selenium-containing protein was 76.03 %. Further studies showed：selenium-enriched soybean not only had selenium-containing protein，but also had other selenium-containing compounds.
文章编号：201603015 中图分类号： 文献标志码：A
基金项目:国家自然科学基金项目（31371085）；辽宁省科技厅项目 （2011205001）
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