Abstract:Bioactive compounds including Vitamin C， betaine， polysaccharides and five main carotenoids in Ningqi No.7 wolfberry fruit were analyzed at different mature stages using HPLC and UV methods. Results indicated that the maximum value of vitamin C and polysaccharide appeared in full ripening fruit， while the peak of betaine content in green ripe stage. Contents of five main carotenoids including β-carotene， lutein， zeaxanthin， β-cryptoxanthin and neoxanthin differed from each other， total carotenoids reached the maximum， then decreased slightly and maintained stable in full ripening stage.