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投稿时间：2014-09-23
投稿时间：2014-09-23
中文摘要: 建立了一种高效液相色谱法同时测定诺尼果汁中车叶草苷酸和去乙酰车叶草苷酸含量的方法。 采用 Shim-pack VP-ODS-C18（250 mm×4.6 mm，5 μm）色谱柱，乙腈-磷酸水溶液为流动相，流速为 0.5 min/mL，检测波长为 237 nm。 在此条件下，车叶草苷酸和去乙酰车叶草苷酸分离良好，浓度分别在 5 μg/mL~50 μg/mL 和 25 μg/mL~250 μg/mL 范围内 线性关系良好，平均加样回收率为 99.02 %和 102.15 %，RSD 值为 1.58 %和 2.40 %。西沙诺尼果汁车叶草苷酸和去乙酰 车叶草苷酸平均含量为 0.204 mg/mL 和 1.640 mg/mL。
Abstract:An analytical method of asperulosidic acid and deacetylasperulosidic acid in Noni Juice was established by HPLC. The separation was performed on a Shim-pack VP-ODS C18 column （250 mm×4.6 mm， 5 μm） with Acetonitrile-phosphoric acid aqueous as mobile phase at flow rate of 0.5 mL/min and detection wave length at 237 nm. Asperulosidic acid and deacetylasperulosidic acid were successfully separated.The calibration curve of asperulosidic acid and deacetylasperulosidic acid were linear in the concentration range of 5 μg/mL-50 μg/mL and 25 μg/mL-250 μg/mL， respectively. The average recoveries of asperulosidic acid and deacetylasperulosidic acid were 99.02 % and 102.15 % ， respectively. The RSD of asperulosidic acid and deacetylasperulosidic acid were 1.58 % and 2.40 %， respectively. The mean content of asperulosidic acid and deacetylasperulosidic acid in Xisha Noni juice were 0.204 mg/mL and 1.640 mg/mL.
keywords: HPLC Xisha Noni Noni juice iridoid compounds asperulosidic acid deacetylasperulosidic acid
文章编号：201602035 中图分类号： 文献标志码：A
基金项目:中国食品发酵工业研究院科技发展基金（博士基金）项目 （KJ14-BS-02）
| 作者 | 单位 |
| 陈建国1，张露1，李雪1，李金霞1，谭望桥2，程池1，* | 1. 中国食品发酵工业研究院，中国工业微生物菌种保藏管理中心，北京 100015； 2.海南诺尼生物工程 开发有限公司，海南 海口 570125 |
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