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投稿时间：2015-09-01
投稿时间：2015-09-01
中文摘要: 将量子点与抗黄曲霉毒素 M1（AFM1）的单克隆抗体偶联，以 AFM1-BSA 偶联物包被于 NC 膜作为检测线，山 羊抗鼠二抗包被于 NC 膜上作为质控线，构建了直接竞争检测 AFM1 的荧光定量免疫层析检测卡体系，在 0.156 μg/L~
1.25 μg/L 范围内能够准确定量黄曲霉毒素 B1，灵敏度达到 0.156 μg/L。
Abstract:By conjugating ployclonal antibody of anti- AFM1 and quantum dots， the system of fluorescent quantitative immunochromatogragphic card was established. The detection line is established by coating NC film with AFM1-BSA conjugate. The detection line is established by coating NC film with sheep anti-rat second antibody. This system could quantified AFM1 correctly in the range of 0.156 μg/L-1.25 μg/L. The sensitivity of the detection card was 0.156 μg/L.
文章编号：201602033 中图分类号： 文献标志码：A
基金项目:天津出入境检验检疫局应用重点项目（TK075-2013）
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