Abstract:This experiment is mainly to pesticide detection pretreatment method was studied， establish proper sample pretreatment method of extraction， purification， concentration， and ensure that when chromatographic determination without the interference of sample matrix， so as to guarantee for the measured results， and prolong the life of chromatographic column， reduce the experiment cost.Mushroom samples with acetonitrile as extraction agent， the vortex oscillation after 10 min， by using C18 SPE powder， PSA SPE， anhydrous magnesium sulfate purification， concentrated bullishness of chromatographic analysis.The recovery rate was above 95 %， RSD under 3 %， the result was satisfactory.The pesticide residue detection pretreatment technology effectively.