Abstract:A high performance liquid chromatography （HPLC） method was developed for the determination of pyraclostrobin residues in Pleurotus ostreatus and Pleurotus citrinopileatus. The degradation kinetics of the pyraclostrobin in Pleurotus ostreatus， Pleurotus citrinopileatus and substrates was thus studied. The results indicated that the half -life period of pyraclostrobin in Pleurotus ostreatus， Pleurotus citrinopileatus and substrates were 2.8， 2.9 d and 14.1 d respectively. Sterilization remarkably reduced pyraclostrobin residues in the substrates. After Pleurotus ostreatus and Pleurotus citrinopileatus were cultivated for 90 days in the substrates with pyraclostrobin， with the tested pesticide residues in the substrates being 0.68 mg/kg， no residual volume was detected in the substance of Pleurotus ostreatus or Pleurotus citrinopileatus that were harvested at the same time. Therefore， pyraclostrobin residues in substrates didn't transfer to the fruiting bodies of Pleurotus ostreatus or Pleurotus citrinopileatus and thus had little impact on the quality safety of the edible fungus.