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投稿时间：2013-12-02
投稿时间：2013-12-02
中文摘要: 研究金银花叶抑菌成分的最佳提取工艺和抑菌效果。以抑菌圈大小为评价指标，通过比较普通回流、超声提取、索氏提取三种方法的效果确定相对较佳提取方法，并在单因素的基础上采用正交试验优化提取工艺，采用梯度稀释法测定提取物的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。结果表明，最佳提取工艺为采用普通回流提取，温度为70 ℃、乙醇体积分数为60 %、料液比为1 ∶ 15 g/mL 的条件下提取时间1.5 h，金银花叶乙醇提取物对大肠杆菌和金黄色葡萄球菌的MIC 分别为500 μg/g 和250 μg/g，MBC 分别为1 000 μg/g 和500 μg/g。结论：采用普通回流提取法能较好地提取金银花叶抑菌活性成分，工艺稳定、可行。
Abstract:To study the optimum extraction process and activity of antibacterial ingredients from Lonicera Japonica Leaves. The inhibition zone as evaluation index， and the normal reflux， ultrasonic extraction and Soxhlet extraction had been compared to obtain the relatively effective extraction method， meanwhile orthogonal experiment had been investigated based on the single factor experiment. In addition， gradient dilution method have been used for researching MIC and MBC. The results showed that， the reflux method was selected at last and the optimum condition of antibacterial ingredients extraction was 60 % ethanol in 1.5 h at 70 ℃and solid to liquid ratio 1 ∶ 15 g/mL. MIC was 250 μg/g and 500 μg/g for Staphylococcus aureus and Escherichia coli，respectively. MBC was 500 μg/g and 1 000 μg/g for Staphylococcus aureus and Escherichia coli， respectively. Conclusion， antibacterial ingredients from Lonicera Japonica Leaves were extracted more fully by the normal reflux， the optimized technology is stable and viable．
keywords: Lonicera japonica leaves effective antibacterial ingredients extraction process antibacterial activity
文章编号：20130736 中图分类号： 文献标志码：
基金项目:国家自然科学基金地区基金项目（31060226）；江西省科技支撑项目（20122BBF60080）
| Author Name | Affiliation |
| LI Shi-chuan | College of Food Science and Engineer， Jiangxi Agricultural University， Nanchang 330045， Jiangxi， China |
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